
Single-cell RNA-sequencing analysis revealed that cdka 1 SLCs show a gene expression profile highly similar to that of sperm cells and not to the generative cell, precursor of the two sperm cells. Here, we first adopt a complementation-based fluorescence-labeling method to successfully separate and collect cdka 1 mutant pollen containing a single SLC. A tool to address this question is the usage of the cyclin-dependent kinase a1 (cdka 1) mutant pollen, which produces a single sperm-like cell (SLC). A long-standing unsolved question is whether the two fertilization events have any preference.



In most angiosperms like Arabidopsis, a pollen grain contains two isomorphic sperm cells required for this double fertilization process. They were not exactly the same,' Ting says, ‘but they always stayed together.' " Ages 6-9.In flowering plants (angiosperms), fertilization of the egg cell by one sperm cell produces an embryo, whereas fusion of a second sperm cell with the central cell generates the endosperm. The final tale, a fantasy spun by Ting, tells of spurning a king who cannot decide which of them to marry: " ‘But the twins told the king to go away. The girls wear identical, feminine dresses, again challenging the reader to look beyond exteriors. Each scenario––ranging from making dumplings to giggling over card tricks gone wrong––is captured in framed, photo-sized illustrations with old-fashioned charm. Snip! Clip! The barber cuts Ling's hair in a smooth line." Not so with Ting, who wiggles, sneezes, and ends up with an asymmetrical hairdo that unquestionably sets her apart. In the first, Ting and Ling get haircuts, but "Ling can always sit still. "Ting laughs because she is thinking exactly the same thing!" Six entertaining tales are blithesome slices of life that exemplify their uniqueness and companionability. "We are not exactly the same," Ling replies to this common comment. Twins will find endearing advocates in Ling and Ting, Chinese-American twins who good-naturedly defy people's assumptions that they are interchangeable.
